The main objective of the current study is to look into the synthesis of zinc oxide (ZnO) quantum dots (QDs) using zinc acetate that is precursor and neem leaf extract as a reducing agent. The synthesis of ZnO QDs was monitored by ultraviolet-visible absorption spectroscopy at wavelength 324 nm. The optimal synthesis of ZnO QDs was recorded at a temperature of 20 degrees and 40 degrees, the physical interaction between ZnO QDs and methyl orange was studied, the interaction between them to check the photo catalytic effect of it. ZnO QDs were applied in rheo leave to see the plasmolysis effect of it, the work opens up the application in several commercial and clinical products, preservatives, and fluorescence labeling including the antimicrobial agents. The plant contains numerous compounds in its body. These biomolecules are produced and stored by plants for their survival, physiological processes, energy, reproduction, safety, etc. interestingly most of these plant-based biomolecules exhibit different medicinal properties and many of these compounds or biomolecules are either excellent drugs or precursor of some important drugs. So, our motive is to find the pharmaceutical importance of the plants and their interaction with the Zn for further research, which can thereby be used for the beneficial needs of humans.